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In an effort to develop efficient seedling propagation system through somatic embryogenesis for the hydroponic culture of Oenanthe stolonifera DC., studies have been conducted on the factors affecting the formation and germination of somatic embryos. Treatment of 0.001 ㎎·L^(-1) TDZ was as effective as 0.1 ㎎·L^(-1) BA in inducing somatic embryos. Regarding the effectivity of the two growth regulators, it was considered that TDZ has more stronger hormonal action than BA. Both of the treatments given for the first 2 weeks of culture from inoculation showed the highest embryo induction and the least abnormal embryo formation. PEG (10.0%) was an effective osmoticum for somatic embryo formation, when used with 0.05 ㎎·L^(-1) ABA. It was found that osmoticum treatment during the first 2 weeks of culture was better than that during the last 2 weeks of culture. Taken collectively, treatment of 0.001 ㎎·L^(-1) TDZ, 10.0% PEG, and 0.05 ㎎·L^(-1) ABA was better than that of any other treatment, when the treatment was given for the first 2 weeks of culture. The optimum callus inoculation density for somatic embryo formation was 30 ㎎ in 15 mL of liquid medium. The optimum size of somatic embryo for germination was determined as 1.0 ㎝ or longer with sufficient cotyledon development of the somatic embryos. The optimum medium for somatic embryo germination was An´s salts which was adopted for the hydroponic culture solution. Tissues of in vitro cultured somatic embryo-derived plants were gradually hardened during acclimatization period and showed similar structures of the seed-propagated plants in a greenhouse.
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